Methods in Biochemistry
Chemistry · 3 hrs Lecture | 3 hrs Lab
Offered: Fall Or Winter, Every Year
CHEM-4506 prerequisites
8 courses appear in this chain, up to 6 levels deep. 7 are needed whichever route you take: CHEM-1111 → CHEM-1112 → BIOL-1115 → CHEM-2202 → CHEM-3502 → CHEM-3503 → CHEM-4502
Part of this course's requisite text couldn't be parsed into structure, so the chain below may be incomplete. The full wording is under “Requisite courses”.
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Pre-Calculus Mathematics 40S
Applied Mathematics 40S
Chemistry 40S
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Chemistry 40S
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Pre-Calculus Math 40S
Applied Math 40S
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Pre-Calculus Math 40S
Applied Math 40S
Chemistry 40S
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Where CHEM-4506 counts
Read from the departments' own calendar pages, where 71 of 141 programs are only partly machine-readable, so treat these as a minimum rather than the full list. Confirm with an academic advisor.
Description
This course examines the application of physical methods to problems of biochemical interest. Topics to be covered include the isolation and stabilization of proteins; sub-cellular fractionation; methods for the assay of protein activity; the determination of enzyme kinetic parameters; purification and analysis of biomolecules (especially proteins) by selective precipitation (varying pH, salt concentration, and solvent), liquid chromatography (ion exchange, gel filtration, and affinity chromatography) and electrophoresis (slab gel and capillary); ultracentrifugation; and biochemical applications of the use of radioisotopes. Although emphasis is placed on the application of these techniques to proteins, application to other classes of biomolecules are examined as appropriate.
Requisite courses
CHEM-3503 [prerequisite(s)]; CHEM-4506L (lab) (must be taken concurrently); CHEM-4502 (recommended prerequisite).
From the 2026-27 undergraduate calendar. Always verify details on WebAdvisor or with an academic advisor before registering.